LOCUS       Exported                4191 bp ds-DNA    circular SYN 17-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0099_pluxstRBS-sfGFP-stRBS-luxI
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Daniel Gerngross
  TITLE     Direct Submission
  JOURNAL   Exported 17 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      1..126
                     /note="T0"
     terminator      27..121
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     misc_feature    127..196
                     /note="spacer (B0040)"
     promoter        197..251
                     /note="lux rR (R0062)"
     RBS             260..271
                     /note="RBS (B0034)"
     CDS             278..1003
                     /codon_start=1
                     /note="sfGFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRP*"
     RBS             1012..1023
                     /note="RBS (B0034)"
     CDS             1030..1614
                     /codon_start=1
                     /note="luxI (C0161)"
                     /translation="MTIMIKKSDFLAIPSEEYKGILSLRYQVFKQRLEWDLVVENNLES
                     DEYDNSNAEYIYACDDTENVSGCWRLLPTTGDYMLKSVFPELLGQQSAPKDPNIVELSR
                     FAVGKNSSKINNSASEITMKLFEAIYKHAVSQGITEYVTVTSTAIERFLKRIKVPCHRI
                     GDKEIHVLGDTKSVVLSMPINEQFKKAVLN*"
     terminator      1626..1712
                     /gene="Escherichia coli rrnB"
                     /note="T1"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1706..1733
                     /note="T1"
     primer_bind     1740..1759
                     /note="AB179"
     primer_bind     1925..1944
                     /note="AB5"
     rep_origin      2078..2905
                     /direction=RIGHT
                     /note="p15A"
     rep_origin      2326..2328
                     /direction=RIGHT
                     /note="P15 ori from pACYC184"
     rep_origin      complement(2329..2873)
                     /direction=LEFT
                     /note="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     primer_bind     2977..2996
                     /note="AB180"
     oriT            3007..3115
                     /note="incP origin of transfer"
     CDS             complement(3271..4086)
                     /note="KamR"
ORIGIN
        1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg
       61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      121 ccaagaaggt tctgttaagt aactgaaccc aatgtcgtta gtgacgctta cctcttaaga
      181 ggtcactgac ctaacaacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat
      241 agtcgaataa atactagaga aagaggagaa atactagatg agcaaaggag aagaactttt
      301 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
      361 tgtccgtgga gagggtgaag gtgatgctac aaacggaaaa ctcaccctta aatttatttg
      421 cactactgga aaactacctg ttccgtggcc aacacttgtc actactctga cctatggtgt
      481 tcaatgcttt tcccgttatc cggatcacat gaaacggcat gactttttca agagtgccat
      541 gcccgaaggt tatgtacagg aacgcactat atctttcaaa gatgacggga cctacaagac
      601 gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaagggtat
      661 tgattttaaa gaagatggaa acattcttgg acacaaactc gagtacaact ttaactcaca
      721 caatgtatac atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg
      781 ccacaacgtt gaagatggtt ccgttcaact agcagaccat tatcaacaaa atactccaat
      841 tggcgatggc cctgtccttt taccagacaa ccattacctg tcgacacaat ctgtcctttc
      901 gaaagatccc aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg
      961 gattacacat ggcatggatg agctctacaa aaggccttaa taatactagt gaaagaggag
     1021 aaatactaga tgactataat gataaaaaaa tcggattttt tggcaattcc atcggaggag
     1081 tataaaggta ttctaagtct tcgttatcaa gtgtttaagc aaagacttga gtgggactta
     1141 gttgtagaaa ataaccttga atcagatgag tatgataact caaatgcaga atatatttat
     1201 gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt tattacctac aacaggtgat
     1261 tatatgctga aaagtgtttt tcctgaattg cttggtcaac agagtgctcc caaagatcct
     1321 aatatagtcg aattaagtcg ttttgctgta ggtaaaaata gctcaaagat aaataactct
     1381 gctagtgaaa ttacaatgaa actatttgaa gctatatata aacacgctgt tagtcaaggt
     1441 attacagaat atgtaacagt aacatcaaca gcaatagagc gatttttaaa gcgtattaaa
     1501 gttccttgtc atcgtattgg agacaaagaa attcatgtat taggtgatac taaatcggtt
     1561 gtattgtcta tgcctattaa tgaacagttt aaaaaagcag tcttaaatta ataaattaaa
     1621 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1681 tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagacagct gggcgcgcca
     1741 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga
     1801 tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca
     1861 ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg
     1921 tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt
     1981 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg
     2041 caaaagcacc gccggacatc agcgctagcg gagtgtatac tggcttacta tgttggcact
     2101 gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc
     2161 agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg
     2221 tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg
     2281 ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct
     2341 ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac
     2401 aggactataa agataccagg cgtttcccct ggcggctccc tcgtgcgctc tcctgttcct
     2461 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct
     2521 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
     2581 tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca
     2641 tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt
     2701 catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc
     2761 agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg
     2821 cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc
     2881 atcttattaa tcagataaaa tatttctagc atgagcggat acatatttga atgtatttag
     2941 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgctagggcc
     3001 ggcctacggc cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg
     3061 gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg
     3121 acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg
     3181 tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc
     3241 agcggaaaag gacccttgtc caattaatta ttagaaaaat tcatccagca tcagatgaaa
     3301 ttgcagtttg ttcatatccg gattatcaat gccatatttc tgaaacagac gtttttgcag
     3361 gctcgggcta aattcgccca ggcagttcca cagaatggcc agatcctgat aacgatccgc
     3421 aatgcccaca cggcccacat caatgcagcc aatcagtttg ccttcatcga aaatcaggtt
     3481 atccaggcta aaatcgccgt gggtcaccac gctatccggg ctaaacggca gcagtttatg
     3541 catttctttc cacacctgtt ccaccggcca gccgttacgt tcatcatcaa aatcgctcgc
     3601 atccaccagg ccgttgttca tacggctctg cgcctgggcc agacgaaaca cacgatcgct
     3661 gttaaacggg cagttgcaca ccggaatgct atgcagacga cgcagaaaca cggccagcgc
     3721 atccacaatg ttttcgccgc tatccggata ttcttccagc acctgaaacg cggttttgcc
     3781 cggaatcgcg gtggtcagca gccacgcatc atccggggtg cgaataaaat gtttaatggt
     3841 cggcagcggc ataaattcgg tcagccagtt cagacgcacc atttcatcgg tcacatcgtt
     3901 cgccacgctg cctttgccat gtttcagaaa cagttccggc gcatccggtt tgccatacag
     3961 acgataaatg gtcgcgccgc tctgacccac gttatcacgc gcccatttat agccatacag
     4021 atccgcatcc atgttgctgt tcagacgcgg acggctacag ctcgtttcac gctgaatatg
     4081 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga
     4141 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaaatt t
//
